Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: p53 , REA1132 , 50 , 130-119-502 , PE , Miltenyi Biotec.

Techniques: Imaging

Journal: Antioxidants

Article Title: Saposhnikovia divaricata Inhibits Inflammation, Oxidative Stress, and Ferroptosis to Alleviate DSS-Induced Ulcerative Colitis

doi: 10.3390/antiox15020258

Figure Lengend Snippet: Network pharmacology analysis identifies p53 as a core ferroptosis-related target of FF in UC. ( A ) Venn diagram illustrating the intersection of FF compound targets with ferroptosis- and UC-related targets. ( B ) Protein–protein interaction (PPI) network of the common targets. Node size and color intensity represent the degree of connectivity, with TP53 (p53) identified as the core target. ( C ) Compound-target-pathway network diagram. The inner pink nodes represent the 38 intersecting targets linking FF, UC, and ferroptosis. ( D ) Gene Ontology (GO) enrichment analysis of the common targets, categorized into Biological Process (BP, red), Cellular Component (CC, green), and Molecular Function (MF, blue). ( E ) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.

Article Snippet: The p53 antibody was obtained from Boster Biological Technology Co., Ltd. (Pleasanton, CA, USA, Item #BM0101).

Techniques:

Journal: Antioxidants

Article Title: Saposhnikovia divaricata Inhibits Inflammation, Oxidative Stress, and Ferroptosis to Alleviate DSS-Induced Ulcerative Colitis

doi: 10.3390/antiox15020258

Figure Lengend Snippet: FF modulates the expression of ferroptosis-related proteins in colon tissue via the p53 pathway. ( A ) Representative immunohistochemical (IHC) images of p53, SLC7A11, and GPX4 expression in colon sections (scale bar = 50 μm). ( B – D ) Quantitative analysis of the relative protein expression levels of p53 (B), SLC7A11 (C), and GPX4 (D). Data are presented as the mean ± SD ( n = 3 independent experiments). ### p < 0.001 versus the control (CON) group; * p < 0.05, ** p < 0.01, *** p < 0.001 versus the DSS model group.

Article Snippet: The p53 antibody was obtained from Boster Biological Technology Co., Ltd. (Pleasanton, CA, USA, Item #BM0101).

Techniques: Expressing, Immunohistochemical staining, Control

Journal: International Journal of Cancer

Article Title: Bcl-2 with loss of apoptosis allows accumulation of genetic alterations: A pathway to metastatic progression in human breast cancer

doi: 10.1002/(sici)1097-0215(20000320)89:2<142::aid-ijc7>3.0.co;2-b

Figure Lengend Snippet: FIGURE 1 – Percentage of tumors with Bcl-2 over-expression according to the number of accumulated oncogene alterations (c-Myc, EGFR and c-erbB-2) and p53 status (mutated vs. non-mutated tumors).

Article Snippet: Bcl-2, c-Myc, c-erbB-2, p53, EGFR and hormone receptor (PR, ER) expression Expression of these genes was assessed by immuno-histochemistry (IHC) using appropriate antibodies: the monoclonal antibody (MAb) anti-Bcl-2 124 (ICI, Cambridge Research Biochemicals, Northwich, UK) at 1:200 dilution; MAb to p53, clone BP-53-12-1 (Biogenex, San Ramon, CA); MAb Ab-6, clone DO-1 (Oncogene Science, Uniondale, NY); MAb Ab-1, clone 9E10 (Oncogene Science), which reacts with human c-Myc at 1:100 dilution; MAb anti-C-erbB-2, clone CB11 (Biogenex) at 1:80 dilution; MAb anti-EGFR, clone E30 (Merck, Darmstadt, Germany) at 1:15 dilution.

Techniques: Over Expression

Journal: International Journal of Cancer

Article Title: Bcl-2 with loss of apoptosis allows accumulation of genetic alterations: A pathway to metastatic progression in human breast cancer

doi: 10.1002/(sici)1097-0215(20000320)89:2<142::aid-ijc7>3.0.co;2-b

Figure Lengend Snippet: FIGURE 2 – Percentage of tumors with p53 over-expression according to the number of accumulated oncogene alterations (c-Myc, EGFR and c-erbB-2) and histological grade (I/II or III).

Article Snippet: Bcl-2, c-Myc, c-erbB-2, p53, EGFR and hormone receptor (PR, ER) expression Expression of these genes was assessed by immuno-histochemistry (IHC) using appropriate antibodies: the monoclonal antibody (MAb) anti-Bcl-2 124 (ICI, Cambridge Research Biochemicals, Northwich, UK) at 1:200 dilution; MAb to p53, clone BP-53-12-1 (Biogenex, San Ramon, CA); MAb Ab-6, clone DO-1 (Oncogene Science, Uniondale, NY); MAb Ab-1, clone 9E10 (Oncogene Science), which reacts with human c-Myc at 1:100 dilution; MAb anti-C-erbB-2, clone CB11 (Biogenex) at 1:80 dilution; MAb anti-EGFR, clone E30 (Merck, Darmstadt, Germany) at 1:15 dilution.

Techniques: Over Expression

Journal: Scientific Reports

Article Title: Sprint-interval but not continuous exercise increases PGC-1α protein content and p53 phosphorylation in nuclear fractions of human skeletal muscle

doi: 10.1038/srep44227

Figure Lengend Snippet: ( a ) Representative immunoblots corresponding to total and phosphorylated protein content measured in the nuclear and cytosolic fractions, before (Pre), immediately (+0 h) and 3 h (+3 h) after the CE and SIE trials. p-p38 MAPK Thr180/Tyr182 : bottom band at ~38 kDa; PHF20: top band at ~105 kDa. No band was detected in the nuclear fractions for p-ACC Ser79 . ( b ) Confirmation of the p53 antibody specificity; samples were run beside an untagged full length p53 recombinant human protein and blotted against both total-p53 (positive control) and p-p53 Ser15 (negative control) antibodies. S: sample; RP: p53 recombinant protein. ( c ) Histone H3 and LDHA were used as indicators of cytosolic and nuclear enrichment, respectively. N: nuclear fractions; C: cytosolic fractions. ( d ) Whole-lane Coomassie blue staining for both nuclear and cytosolic fractions, and ( e ) histone H3 (nuclear) and GAPDH (cytosolic), were used to verify equal loading between lanes. The immunoblot and whole-lane Coomassie images in this figure were cropped to improve the conciseness and clarity of the presentation.

Article Snippet: The specificity of the p53 antibody was assessed by blotting samples beside a commercially-available, untagged, full-length p53 recombinant human protein (Aviva Systems Biology, AEP00002) ( ).

Techniques: Western Blot, Recombinant, Positive Control, Negative Control, Staining

Journal: Scientific Reports

Article Title: Sprint-interval but not continuous exercise increases PGC-1α protein content and p53 phosphorylation in nuclear fractions of human skeletal muscle

doi: 10.1038/srep44227

Figure Lengend Snippet: Protein content of nuclear ( a ) and cytosolic ( b ) p53, of nuclear ( c ) and cytosolic ( d ) PHF20, and of nuclear ( e ) and cytosolic ( f ) p-p53 Ser15 before (Pre), immediately (+0 h) and 3 h (+3 h) after the CE and SIE trials. Open circles (CE) and closed triangles (SIE) represent individual values. Primary effects were analysed using two-way ANOVA with repeated measures for time followed by Tukey’s honestly significant difference post-hoc test for pairwise comparisons. * P < 0.05 vs . Pre; ‡ P < 0.05 vs . CE at the same time point. Individual data points and mean bars are plotted. n = 9 for each type of exercise.

Article Snippet: The specificity of the p53 antibody was assessed by blotting samples beside a commercially-available, untagged, full-length p53 recombinant human protein (Aviva Systems Biology, AEP00002) ( ).

Techniques:

Journal: Scientific Reports

Article Title: Sprint-interval but not continuous exercise increases PGC-1α protein content and p53 phosphorylation in nuclear fractions of human skeletal muscle

doi: 10.1038/srep44227

Figure Lengend Snippet: p53 ( a ), and PGC-1α ( b ) mRNA content before (Pre), immediately (+0 h), and 3 h (+3 h) after the CE and SIE trials, expressed relative to control (cyclophilin, glyceraldehyde 3-phosphate dehydrogenase [GAPDH], and beta-2-microglobulin [B2M] housekeeping genes). Open circles (CE) and closed triangles (SIE) represent individual values. Primary effects were analysed using two-way ANOVA with repeated measures for time followed by Tukey’s honestly significant difference post-hoc test for pairwise comparisons. * P < 0.05 vs . Pre. Individual data points and mean bars are plotted. n = 9 for each type of exercise.

Article Snippet: The specificity of the p53 antibody was assessed by blotting samples beside a commercially-available, untagged, full-length p53 recombinant human protein (Aviva Systems Biology, AEP00002) ( ).

Techniques: Control

Journal: Scientific Reports

Article Title: Sprint-interval but not continuous exercise increases PGC-1α protein content and p53 phosphorylation in nuclear fractions of human skeletal muscle

doi: 10.1038/srep44227

Figure Lengend Snippet: Primers used for real-time RT-PCR analyses of mRNA expression.

Article Snippet: The specificity of the p53 antibody was assessed by blotting samples beside a commercially-available, untagged, full-length p53 recombinant human protein (Aviva Systems Biology, AEP00002) ( ).

Techniques: Quantitative RT-PCR, Expressing